NanoTI02 (UV-Titan) does not induce ESTR mutations in the germline of prenatally exposed female mice

Tidsskriftartikel - 2012

Resume

[Open access] Particulate air pollution has been linked to an increased risk of cardiovascular disease and cancer. Animal studies have shown that inhalation of air particulates induces mutations in the male germline. Expanded simple tandem repeat (ESTR) loci in mice are sensitive markers of mutagenic effects on male germ cells resulting from environmental exposures; however, female germ cells have received little attention. Oocytes may be vulnerable during stages of active cell division (e.g., during fetal development). Accordingly, an increase in germline ESTR mutations in female mice prenatally exposed to radiation has previously been reported. Here we investigate the effects of nanoparticles on the female germline. Since pulmonary exposure to nanosized titanium dioxide (nanoTiO2) produces a long-lasting inflammatory response in mice, it was chosen for the present study. Findings Pregnant C57BL/6 mice were exposed by whole-body inhalation to the nanoTiO2 UV-Titan L181 (~42.4 mg UV-Titan/m3) or filtered clean air on gestation days (GD) 8-18. Female C57BL/6 F1 offspring were raised to maturity and mated with unexposed CBA males. The F2 descendents were collected and ESTR germline mutation rates in this generation were estimated from full pedigrees (mother, father, offspring) of F1 female mice (192 UV-Titan-exposed F2 offspring and 164 F2 controls). ESTR mutation rates of 0.029 (maternal allele) and 0.047 (paternal allele) in UV-Titan-exposed F2 offspring were not statistically different from those of F2 controls: 0.037 (maternal allele) and 0.061 (paternal allele).

Reference

Boisen A, Shipley T, Jackson P, Hougaard KS, Wallin EHR, Yauk C, Vogel UB. NanoTI02 (UV-Titan) does not induce ESTR mutations in the germline of prenatally exposed female mice. Particle and Fibre Toxicology 2012;9(19):.
doi: 10.1186/1743-8977-9-19

Gå til Tidsskriftartikel

Relaterede projekter

Dansk Center for Nanosikkerhed Arvelige mutationer efter prænatal udsættelse for nanopartikler