Artboard 2 copy 35Artboard 64 copy 13Artboard 2 copy 19Artboard 2 copy 31Artboard 64 copy 18Artboard 64 copy 10Artboard 64 copy 11Artboard 64 copy 15Artboard 64 copy 12Artboard 64 copy 13Artboard 64 copy 14Artboard 2 copy 34Artboard 64 copy 19Artboard 64 copy 16MinusArtboard 2 copy 44Artboard 2 copy 38Artboard 2 copy 36PlusArtboard 64 copy 17Artboard 2 copy 43Artboard 2 copy 45Artboard 2 copy 46Artboard 64 copy 16Artboard 64 copy 18Artboard 64 copy 19Artboard 64 copy 17

Interaction of biologically relevant proteins with ZnO nanomaterials: A confounding factor for in vitro toxicity endpoints

Tidsskriftartikel - 2019

Resume

The results of in vitro toxicological studies for manufactured nanomaterials (MNs) are often contradictory and not reproducible. Interference of the MNs with assays has been suggested. However, understanding for which materials and how these artefacts occur remains a major challenge. This study investigated interactions between two well-characterized ZnO MNs (NM-110 and NM-111) and lactate dehydrogenase (LDH), and two interleukins (IL-6 and IL-8). Particles (10 to 640 μg/mL) and proteins were incubated for up to 24 h in routine in vitro assays test conditions. LDH activity (OD-LDH), but not interleukins concentrations, decreased sharply in a dose-dependent manner within an hour after exposure (OD-LDH < 60% of ODref for both MNs at 10 μg/mL). A Freundlich adsorption isotherm was successfully applied, indicating multilayer adsorption of LDH. ZnO MNs and LDH had neutral to slightly negative surface charges in dispersion, precluding electrostatic attachment. Particle sedimentation was not a limiting factor. Fast dissolution of ZnO MNs was shown and Zn2+ could play a role in the OD-LDH drop. To summarize, ZnO MNs quickly reduced OD-LDH due to concentration-dependent adsorption and LDH inhibition by interaction with dissolved Zn. The control of particle interference in toxicological in vitro assays should become mandatory to avoid misleading interpretation of results.

Reference

Da Silva E, Kembouche Y, Tegner U, Baun A, Jensen KA. Interaction of biologically relevant proteins with ZnO nanomaterials: A confounding factor for in vitro toxicity endpoints. Toxicol In Vitro 2019;56:41-51.
doi: 10.1016/j.tiv.2018.12.016

Gå til Tidsskriftartikel

Relaterede projekter

NANoREG