Airway irritation, inflammation and toxicity in mice following inhalation of metal oxide nanoparticles

Tidsskriftartikel - 2016


Metal oxide nanoparticles are used in a broad range of industrial processes and workers may be exposed to aerosols of the particles both during production and handling. Despite the widespread use of these particles, relatively few studies have been performed to investigate the toxicological effects in the airways following inhalation. In the present study, the acute (24 h) and persistent (13 weeks) effects in the airways after a single exposure to metal oxide nanoparticles were studied using a murine inhalation model. Mice were exposed 60 minutes to aerosols of either ZnO, TiO2, Al2O3 or CeO2 and the deposited doses in the upper and lower respiratory tracts were calculated. Endpoints were acute airway irritation, pulmonary inflammation based on analyses of bronchoalveolar lavage (BAL) cell composition, DNA damage assessed by the comet assay and pulmonary toxicity assessed by protein level in BAL fluid and histology. All studied particles reduced the tidal volume in a concentration-dependent manner accompanied with an increase in the respiratory rate. In addition, ZnO and TiO2 induced nasal irritation. BAL cell analyses revealed both neutrophilic and lymphocytic inflammation 24 h post exposure to all particles except TiO2. The ranking of potency regarding induction of acute lung inflammation was Al2O3= TiO2


Larsen ST, Jackson P, Poulsen SS, Levin DHM, Jensen KA, Wallin EHR, Nielsen GD, Koponen IK. Airway irritation, inflammation and toxicity in mice following inhalation of metal oxide nanoparticles. Nanotoxicology 2016;10(9):1254-1262.
doi: 10.1080/17435390.2016.1202350

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