Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise

Tidsskriftartikel - 2009

Resume

Despite the widespread consumption of nonsteroidal anti-inflammatorydrugs (NSAIDs), the influence of these drugs on muscle satellitecells is not fully understood. The aim of the present studywas to investigate the effect of a local NSAID infusion on satellitecells after unaccustomed eccentric exercise in vivo in humanskeletal muscle. Eight young healthy males performed 200 maximaleccentric contractions with each leg. An NSAID was infused viaa microdialysis catheter into the vastus lateralis muscle ofone leg (NSAID leg) before, during, and for 4.5 h after exercise,with the other leg working as a control (unblocked leg). Musclebiopsies were collected before and 8 days after exercise. Changesin satellite cells and inflammatory cell numbers were investigatedby immunohistochemistry. Satellite cells were identified usingantibodies against neural cell adhesion molecule and Pax7. Thenumber of Pax7+ cells per myofiber was increased by 96% on day8 after exercise in the unblocked leg (0.14 ± 0.04, mean± SE) compared with the prevalue (0.07 ± 0.02,P <0.05), whereas the number of Pax7+ cells was unchangedin the leg muscles exposed to the NSAID (0.07 ± 0.01).The number of inflammatory cells (CD68+ or CD16+ cells) wasnot significantly increased in either of the legs 8 days afterexercise and was unaffected by the NSAID. The main finding inthe present study was that the NSAID infusion for 7.5 h duringthe exercise day suppressed the exercise-induced increase inthe number of satellite cells 8 days after exercise. These resultssuggest that NSAIDs negatively affect satellite cell activityafter unaccustomed eccentric exercise.

Reference

Mikkelsen U, Langberg H, Helmark I, Skovgaard D, Andersen LL, Kjær M, Mackey A. Local NSAID infusion inhibits satellite cell proliferation in human skeletal muscle after eccentric exercise. Journal of Applied Physiology 2009;107(5):1600-1611.
doi: 10.1152/japplphysiol.00707.2009

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